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OrganRXTM Tissue builds quality, transplantable tissue for organ repair needs. Using the advanced biomimetic OrganRXTM technologies allows for the construction of functional organ tissue for therapeutic discoveries.
The multi-organ recirculation system (OrganRXTM) provides passive gravity-driven unidirectional shear flow to multi-organ culture. The OrganRXTM plate can accommodate multiple micro-organs such as liver, gut, kidney and brain.
The organ plate is an injection molded glass bottom disposable plate. The users can populate the plate with primary cells, cell lines or IPS cells and mature organs by media recirculation. Currently the plate has six multi-organ units.
We provide testing of compounds in 2-D and 3-D blood-brain-barrier tissue models. OrganRXTM enables determination of transport characteristics of novel compounds to/from the brain and other organs. - Applications
Applications
OrganRXTM helps to recapitulate human organs with physiological, mechanical and biochemical complexities and their interaction through the exchange of metabolites or signaling molecules and selective transport across the barriers.
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LC-MS analysis of drug metabolism using OrganRXTM System
As a drug enters the body, its dynamics and metabolism can affect its final effects and even induce off-target effects that impact tissues and organs. To model drug dynamics in the microenvironment, OrganRXTM is used with multiple cell types forming a multi-organ system. As specific organs such as the liver or gut metabolize a drug, the effects of the metabolites on the other organs can be observed as modeled on the OrganRXTM system. Liquid chromatography-mass spectroscopy (LC-MS) is an analytical tool used to measure drug metabolism, offering a more detailed analysis of the effects of drugs on the organ system.
LC-MS characterizes biomarkers from the cell media before and after applying a drug to reveal the metabolites of the drug produced by specific tissues. LC-MS is advantageous for identifying metabolites at high sensitivity down to nanomolar and high-throughput analysis.
Currently, LC-MS analysis of drug metabolism in OrganRXTM is done by removing cell media and analyzing the drug consumption and production of metabolites by each organ. LC-MS sampling with OrganRXTM can provide faster and more accurate results for high throughput assays such as toxicity assays.
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