OrganRX™ CRO Services

TUMOR MICROENVIRONMENT FOR THERAPEUTIC SCREENING

Using the OrganRX™ Plate and OrganRX™ System, cell-cell and cell-matrix interactions within 3D tumor spheroid models are studied to provide deeper insights into drug response and tumor behavior. The OrganRX™ Plate supports co-culture of tumor spheroids/organoids with stromal, endothelial, and immune cells and can model multiple replicates of multi-organ interactions on a single plate to give early efficacy and toxicity insight. For the the TME therapeutic screening, Immuno-oncology (IO) cells such as activated T-Cells are introduced into the outer wells while tumor spheroids reside in the inner channels; the T-cells can be recirculated using the OrganRX™ System, collected and analyzed to measure activation markers by flow cytometry or by endpoint immunofluorescence on cancer cells.

Built for throughput and rich functional readouts, OrganRX™ integrates non-invasive live imaging with standard endpoint assays so you can prioritize translational candidates before moving into animal or clinical studies. Typical functional readouts include live-cell imaging of T-cell infiltration and apoptosis,tumor viability assays, flow cytometry on recirculated IO cells, or multiplex cytokine and chemokine profiling.

As an example, shown below is the live cell imaging of Ipilimumab (mAb) and HLA-matched T-cells (with CellTracker Green) infiltrating human liver spheroids captured within the OrganRX™ Plate.