Legend
2. User Input
3. Calculated Output
4. Intermediate Values (cannot be edited)
6. Number of units per plate
7. No of plates
8. Liver
0
1
9. Kidney
0
1
10. Gut
0
1
11. Brain
0
1
12. Vascular Liver
0
1
13. Vascular Kidney
0
1
14. Vascular Gut
0
1
15. Vascular Brain
0
1
16. Gel volume tolerance percentage
%
17. Cell density tolerance percentage
%
Fibrinogen + Thrombin gel for Organs
Liver
22. Volume of Fibrinogen/Thrombin/cell gel
ul
23. Tolerance volume
ul
24. Volume of gel mix per plate
ul
25. Total gel for all plates
ul
Kidney
28. Volume of Fibrinogen/Thrombin/cell gel
ul
29. Tolerance volume
ul
30. Volume of gel mix per plate
ul
31. Total gel for all plates
ul
Gut
34. Volume of Fibrinogen/Thrombin/cell gel
ul
35. Tolerance volume
ul
36. Volume of gel mix per plate
ul
37. Total gel for all plates
ul
Brain
40. Volume of Fibrinogen/Thrombin/cell gel
ul
41. Tolerance volume
ul
42. Volume of gel mix per plate
ul
43. Total gel for all plates
ul
Vascular Region
46. Volume of Liver-Endothelial region
ul
47. Volume of Kidney-Endothelial region
ul
48. Volume of Gut-Endothelial region
ul
49. Volume of Brain-Endothelial region
ul
50. Total Endothelial cell gel volume
ul
52. Total Gel Needed
ul
Working Fibrinogen Solution
55. Phenol red color factor
56. Required total Fibrinogen gel working solution
ul
57. Fibrinogen working solution concentration
mg/ml
58. Mass of phenol red to add to PBS
mg
59. Volume of PBS with phenol red
ul
60. Fibrinogen powder
mg
Stock solution of Thrombin
63. Number of NIH Units
U
64. U/mg Solid
U/mg
65. Solid in bottle
mg
66. Units in bottle
U
67. Desired concentration of Thrombin stock solution
U/ml
68. Desired volume of stock solution
ml
69. Thrombin stock solution concentration in mg/ml
mg/ml
Thrombin Working solution
72. Required total thrombin gel working solution
ul
73. Required thrombin concentration
U/ml
74. Thrombin working solution
ul
75. Volume of heparin medium for required concentration
ul
76. Heparin-Thrombin solution
ul
Cells Available from T flasks
79. Number of Liver cells from T- flask
cells
80. Number of flasks
81. Total Number of Liver cells
cells
83. Number of Kidney cells from T-75 flask
cells
84. Number of flasks
85. Total Number of Kidney cells
cells
87. Number of Gut cells from T-75 flask
cells
88. Number of flasks
89. Total Number of Gut cells
cells
91. Number of Brain cells from T-75 flask
cells
92. Number of flasks
93. Total Number of Brain cells
cells
95. Number of Endothelial cells from T75 flask
cells
96. Number of flasks
97. Total number of Endothelial cells
cells
99. Number of Fibroblast cells from T75 flask
cells
100. Number of flasks
101. Total number of Fibroblast cells
cells
Liver
105. Desired cell density for organ in channel
cells/ul
106. Cells required for the experiment
cells
107. Resuspension cell density in tube
cells/ul
108. Resuspension media volume in the tube
ul
109. Volume of cells for thrombin working solution
ul
110. Volume of cell resuspension left
ul
111. Cells remaining
cells
112. Volume of thrombin working solution
ul
113. Volume of fibrinogen solution
ul
Kidney
116. Desired cell density for organ in channel
cells/ul
117. Cells required for the experiment
cells
118. Resuspension cell density in tube
cells/ul
119. Resuspension media volume in the tube
ul
120. Volume of cells for thrombin working solution
ul
121. Volume of cell resuspension left
ul
122. Cells remaining
cells
123. Volume of thrombin working solution
ul
124. Volume of fibrinogen solution
ul
Gut
127. Desired cell density for organ in channel
cells/ul
128. Cells required for the experiment
cells
129. Resuspension cell density in tube
cells/ul
130. Resuspension media volume in the tube
ul
131. Volume of cells for thrombin working solution
ul
132. Volume of cell resuspension left
ul
133. Cells remaining
cells
134. Volume of thrombin working solution
ul
135. Volume of fibrinogen solution
ul
Brain
138. Desired cell density for organ in channel
cells/ul
139. Cells required for the experiment
cells
140. Resuspension cell density in tube
cells/ul
141. Resuspension media volume in the tube
ul
142. Volume of cells for thrombin working solution
ul
143. Volume of cell resuspension left
ul
144. Cells remaining
cells
145. Volume of thrombin working solution
ul
146. Volume of fibrinogen solution
ul
Endothelial cells / Fibroblast for Outer Channels
149. Desired cell density for organ in channel
cells/ul
150. Cells required for the experiment
cells
151. Resuspension cell density in tube
cells/ul
152. Resuspension media volume in the tube
ul
153. Volume of cells for thrombin working solution
ul
154. Volume of cell resuspension left
ul
155. Cells remaining
cells
156. Volume of thrombin working solution
ul
157. Volume of fibrinogen solution
ul
Name
E-mail Id
The multi-organ recirculation system (OrganRXTM) provides passive gravity-driven unidirectional shear flow to multi-organ culture. The OrganRXTM plate can accommodate multiple micro-organs such as liver, gut, kidney and brain.
The organ plate is an injection molded glass bottom disposable plate. The users can populate the plate with primary cells, cell lines or IPS cells and mature organs by media recirculation. Currently the plate has six multi-organ units.
We provide testing of compounds in 2-D and 3-D blood-brain-barrier tissue models. OrganRXTM enables determination of transport characteristics of novel compounds to/from the brain and other organs.
