BBB Form

Online Admission Registration

BBB ORGAN

Name

E-mail Id

Number of units per plate

Materials for making Fibrinogen gel

Fibrinogen working solution concentration

Fibrinogen powder

PBS

Materials for making Thrombin gel

Stock solution of Thrombin

NIH U/mg Protein

U/mg Solid

Solid in bottle

Units in bottle

Concentration of Thrombin liquid after dissolving in 1ml PBS

Required thrombin concentration

For making 100U/ml Thrombin stock solution

Thrombin Stock Solution

PBS

Thrombin gel prepration

Volum of Low heparin medium

Volume of thrombin Stock solution

Concentration

Fibrinogen gel + Thrombin gel

Fibrinogen gel solution

Thrombin gel solution

Fibrinogen + Thrombin gel mix per unit

Number of units

Volume of bbb unit

Tolerance

Fibrinogen + Thrombin gel mix per plate

Prepartion of 3d gel and cells

Number of units in OrganRX Plate used for endothelial cells

Number of units in OrganRX Plate used for pericytes

Volume of Cell Suspension for

Endothelial cell ratio

Cell culture

Number of endothelial cells from T-25 flask

Number of flasks

Total Number of endothelial cells

Number of pericytes from T25 flask

Number of flasks

Total number of pericytes

Endothelial cells + Pericyes alone

Desired number of endothelial cells per BBB

Number of endothelial cells per Plate

Cell density in thrombin gel per BBB

Resuspension endothelial cell density in media

Resuspension volume (media)

Volume of cell resuspension for thrombin

Volume of cell resuspension left

Endothelial Cells remaining

Desired number of pericytes per BBB

Number of pericytes per Plate

Cell density in thrombin gel per BBB

Resuspension cell density in media

Resuspension volume (media)

Volume of cell resuspension for fibrogen

Volume of cell resuspension left

Pericytes remaining

Endothelial cells

Desired number of endothelial cells per BBB

Number of endothelial cells per Plate

Volume of endothelial cell resuspension left

Endothelial cell resuspension density

Number of endothelial cells left

Cell density in collagen gel per unit

Resuspension cell density in media

Resuspension Volume (media)

Volume of endothelial cell resuspension for collagen

Volume of cell resuspension left

Remaing cells in tube 1

BBB ORGAN

Stock solution of Thrombin

Prepartion of 3d gel and cells

Cell culture

Endothelial cells + Pericyes alone

Endothelial cells

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